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KMID : 0613820080180071005
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Journal of Life Science
2008 Volume.18 No. 7 p.1005 ~ p.1010
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Inhibitory Effects of Four Solvent Fractions of Alnus firma on α-Amylase and α-Glucosidase
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Choi Hye-Jung
Jeong Yong-Kee
Kang Dae-Ook
Joo Woo-Hong
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Abstract
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In this study, we investigated the inhibitory effect of four solvent fractions of Alnus firma on α-amylase, α-glucosidase and aldose reductase activities. The inhibitory test showed that methanol (MeOH) extract and hexane (HX) fraction strongly inhibited pork pancreatin and salivary α-amylase activity. The MeOH extract and HX fraction of Alnus firma at the concentration of 4 ㎎/ml inhibited more than 70% of pancreatin and salivary α-amylase activity. The inhibitory effect of fractions has different specificities against α-amylase from pancreatin and salivary. In addition, the MeOH extract and butanol (BuOH) fraction showed the highest inhibitory activity on yeast α-glucosidase at values of IC50 137.36 ㎍/ml and 115.14 ㎍/ml respectively. The MeOH extract and BuOH fraction showed the highest inhibitory activity on yeast α-glucosidase than commercial agent such as 1-deoxynorjirimycin and acarbose. Inhibition kinetics of solvent fractions showed that α-glucosidase has been inhibited noncompetitively by the MeOH, EA and BuOH fraction. The aldose reductase from human muscle cell had been inhibited strongly by the MeOH extract and EA fraction at 57.996% and 83.293% at the concentration of 50 ㎍/ml, respectively. These findings may contribute to biological significance in that α-amylase, α-glucosidase and aldose reductase inhibitory compounds could be used as a functional food and a drug for the symptomatic treatment of antidiabetic disease in the future.
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KEYWORD
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Alnusfirma, solventfractions, α-amylase, α-glucosidase, aldosereductase, inhibitoryeffects
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